Course Information:
Course name: Technology of biochemistry and molecular biology
Course number: S041106
Course start time: Spring
Number of classes: 72
Teaching mode: lecture, practice
Teacher in charge: Liudi Yuan
Contact Number: 83272474
Course Introduction:
This course is designed to help the students grasp advanced technology of biochemistry and molecular biology. This course contains two major parts. The first part of this course is genetic engineering and the second part is protein purification. All the hands-on experiments are connected to mimic areal research project, which allow the students to have a systematic view about biochemical researches.
Agenda details:
A.Purification ofproteins:
globulin precipitation through ammonium sulfate.
removal salt by gel filtration.
DEAE-chromatograph
Electrophoresis.
B.Gene engineering
Ligation of gene and plasmid.
Preparation of competent cell.
transfection of recombinant plasmid into E.coli.
identification of the recombinant plasmid.
expression and identification of the protein.
Teaching Schedule:
Week | Course Content | Teaching Method |
1 | Ligation of gene and plasmid. | lecture , practice |
2 | Preparation of competent cell | lecture, practice |
3 | transfection of recombinant plasmid into E.coli | lecture, practice |
4 | identification of the recombinant plasmid. | lecture, practice |
5 | expression of the protein | lecture, practice |
6 | identification of the protein. | lecture, practice |
7 | globulin precipitation through ammonium sulfate. | lecture, practice |
8 | removal salt by gel filtration. | lecture, practice |
9 | DEAE-chromatograph | lecture, practice |
10 | Electrophoresis. | lecture, practice |
