方可 讲师 硕士生导师
E-mail: kfang@seu.edu.cn
办公地点：李文正楼北235
个人简介：
2012年和2018年于中山大学生命科学学院获得学士和博士学位，2018-2021年于中山大学生命科学学院进行博士后研究。2021年加入东南大学生命科学与技术学院，任讲师，硕士生导师。共发表SCI论文20余篇，其中以第一或通讯作者发表在高水平期刊如Cell death and differentiation、 Genome Biology、Journal of Hematology & Oncology 、Clinical and Translational Medicine上。主持国家自然科学基金面上项目、国家自然科学基金青年基金、中国博士后面上项目(一等资助)，入选江苏省“双创博士”和东南大学“至善学者”人才计划。
研究方向：表观遗传及转录调控在细胞命运决定中的功能机制
1. 表观遗传及转录调控介导细胞命运决定的功能机制
基因表达的改变是细胞命运决定及个体命运决定最关键的途径。特定基因座位的表观遗传改变，进而介导的转录差异是细胞命运决定的核心。我们致力于探究基因座位表观遗传的改变（如结构性改变（G4/R-loop），修饰性改变等(组蛋白修饰等)）与基因转录的关系，并在发育（如心脏发育）及疾病（如癌症）模型中探究其对细胞命运决定的调控作用。
2. 转录调控在疾病中的功能机制及靶向治疗
癌基因，抑癌基因等的转录调控异常往往直接介导的癌症产生。我们聚焦于癌症相关基因的转录调控，寻找癌症发生发展的重要调控因子，揭示其细胞功能，如不同细胞死亡调控的切换等。并以此为基础开发潜在的靶向治疗策略。
发表论文：
1.Zhao X*, Fang K*, Liu X*, Yao R, Wang M, Li F, Hao S, He J, Wang Y, Fan M, Huang W, Li Y, Gao C, Lin C, Luo Z. QSER1 Preserves the Suppressive Status of the Pro-Apoptotic Genes to Prevent Apoptosis[J]. Cell Death and Differentiation, 2023, 30(3):779-793.
2.Huang W, Zeng ZC, Wang WT, Sun YM, Chen YQ, Luo XQ, Fang K#. A CRISPR/CAS9-based strategy targets the personalized chimeric neosequence in fusion-driven cancer genome for precision medicine. Clinical and Translational Medicine 2021, 11(3): e355.
3.Fang K*, Huang W*, Sun YM, Chen TQ, Zeng ZC, Yang QQ, Pan Q, Han C, Sun LY, Luo XQ, Wang WT, Chen YQ. Cis-acting lnc-eRNA SEELA directly binds histone H4 to promote histone recognition and leukemia progression. Genome Biology 2020, 21(1): 269.
4.Huang W*, Fang K*, Chen TQ, Zeng ZC, Sun YM, Han C, Sun LY, Chen ZH, Yang QQ, Pan Q, Luo XQ, Wang WT, Chen YQ. circRNA circAF4 functions as an oncogene to regulate MLL-AF4 fusion protein expression and inhibit MLL leukemia progression. J HEMATOL ONCOL 2019, 12(1): 103.
5. Meng S, Liu X, Zhu S, Xie P, Fang H, Pan Q, Fang K, Li F, Zhang J, Che Z, Zhang Q, Mao G, Wang Y, Hu P, Chen K, Sun F, Xie W, Luo Z, Lin C. Young LINE-1 Transposon 5' UTRs Marked by Elongation Factor ELL3 Function as Enhancers to Regulate Naive Pluripotency in Embryonic Stem Cells[J]. Nature Cell Biology, 2023, 25(9):1319-1331
6.Che Z, Liu X, Dai Q, Fang K, Guo C, Yue J, Fang H, Xie P, Luo Z, Lin C. Distinct Roles of the Two SEC Scaffold Proteins, AFF1 and AFF4, in Regulating RNA Pol II Transcription Elongation[J]. Journal of Molecular Cell Biology, 2023.
7.Zeng Z C, Pan Q, Sun Y M, Huang H J, Chen X T, Chen T Q, He B, Ye H, Zhu S X, Pu K J, Fang K, Huang W, Chen Y Q, Wang W T. METTL3 Protects METTL14 From STUB1-mediated Degradation to Maintain M(6) a Homeostasis[J]. Embo Reports, 2023, 24(3):e55762.
8. Han C, Sun LY, Luo XQ, Pan Q, Sun YM, Zeng ZC, Chen TQ, Huang W, Fang K, Wang WT, Chen YQ. Chromatin-associated orphan snoRNA regulates DNA damage-mediated differentiation via a non-canonical complex. CELL REP 2022, 38(13): 110421.
9.Huang W, Chen TQ, Fang K, Zeng ZC, Ye H, Chen YQ. N6-methyladenosine methyltransferases: functions, regulation, and clinical potential. J HEMATOL ONCOL 2021, 14(1): 117. (中科院一区；IF=17.4，五年IF=12.6)
10. Sun L, Wang W, Han C, Huang W, Sun Y, Fang K, Zeng Z, Yang Q, Pan Q, Chen T, Luo X, Chen Y. The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation. MOL CELL 2021, 81(21): 4493-4508.
11. Chen ZH, Chen TQ, Zeng ZC, Wang D, Han C, Sun YM, Huang W, Sun LY, Fang K, Chen YQ, Luo XQ, Wang WT. Nuclear export of chimeric mRNAs depends on an lncRNA-triggered autoregulatory loop in blood malignancies. CELL DEATH DIS 2020, 11(7): 566.
12. Wang WT, Chen TQ, Zeng ZC, Pan Q, Huang W, Han C, Fang K, Sun LY, Yang QQ, Wang D, Luo XQ, Sun YM, Chen YQ. The lncRNA LAMP5-AS1 drives leukemia cell stemness by directly modulating DOT1L methyltransferase activity in MLL leukemia. J HEMATOL ONCOL 2020, 13(1): 78.
13. Sun YM, Wang WT, Zeng ZC, Chen TQ, Han C, Pan Q, Huang W, Fang K, Sun LY, Zhou YF, Luo XQ, Luo C, Du X, Chen YQ. circMYBL2, a circRNA from MYBL2, regulates FLT3 translation by recruiting PTBP1 to promote FLT3-ITD AML progression. BLOOD 2019, 134(18): 1533-1546.
14.Wang WT, Han C, Sun YM, Chen ZH, Fang K, Huang W, Sun LY, Zeng ZC, Luo XQ, Chen YQ. Activation of the Lysosome-Associated Membrane Protein LAMP5 by DOT1L Serves as a Bodyguard for MLL Fusion Oncoproteins to Evade Degradation in Leukemia. CLIN CANCER RES 2019, 25(9): 2795-2808.
15.Sun LY, Li XJ, Sun YM, Huang W, Fang K, Han C, Chen ZH, Luo XQ, Chen YQ, Wang WT. LncRNA ANRIL regulates AML development through modulating the glucose metabolism pathway of AdipoR1/AMPK/SIRT1. MOL CANCER 2018, 17(1): 127.
16.Huang W, Wang WT, Fang K, Chen ZH, Sun YM, Han C, Sun LY, Luo XQ, Chen YQ. MIR-708 promotes phagocytosis to eradicate T-ALL cells by targeting CD47. MOL CANCER 2018, 17(1):
17.Chen ZH, Wang WT, Huang W, Fang K, Sun YM, Liu SR, Luo XQ, Chen YQ. The lncRNA HOTAIRM1 regulates the degradation of PML-RARA oncoprotein and myeloid cell differentiation by enhancing the autophagy pathway. CELL DEATH DIFFER 2017, 24(2): 212-224.
18.Fang K*, Han BW*, Chen ZH, Lin KY, Zeng CW, Li XJ, Li JH, Luo XQ, Chen YQ. A distinct set of long non-coding RNAs in childhood MLL-rearranged acute lymphoblastic leukemia: biology and epigenetic target. HUM MOL GENET 2014, 23(12): 3278-3288.
19.Duan FT, Qian F, Fang K, Lin KY, Wang WT, Chen YQ. miR-133b, a muscle-specific microRNA, is a novel prognostic marker that participates in the progression of human colorectal cancer via regulation of CXCR4 expression. MOL CANCER 2013, 12: 164.
20.Fang K, Qian F, Chen YQ. MicroRNAs as regulators in normal hematopoietic and leukemia stem cells: current concepts and clinical implications. Current Molecular Medicine 2012 Jun,12(5):536-46.
